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A blood culture is usually taken by venipuncture. Venipuncture is done first by painting the skin over the veins in the bend of the elbow with tincture of iodine or other suitable disinfectant like 70 percent alcohol. Some persons may be allergic to iodine. Make a second application of tincture of iodine or 70 percent alcohol to thoroughly sterilize the area. Then compress the area. Secure a tourniquet, but not too tightly, around the arm just above the elbow. Ask the patient to close and open the hand several times.

Puncture a prominent vein with the use of a 20 or 21 gauge needle to which may be attached a 20 ml. syringe or vacutainer. Vacutainer is a specially designed holder for a vacuum tube. Withdraw from 10 to 15 ml. of blood or whatever amount is indicated for the particular laboratory test.

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